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    DIG-High Prime DNA Labeling and Detection Starter Kit II

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    產品名稱: DIG-High Prime DNA Labeling and Detection Starter Kit II
    產品型號: 11585614910
    產品展商: Roche
    產品文檔: 無相關文檔

    簡單介紹

    For 12 random-primed DNA labeling reactions with DIG-11-dUTP, alkali-labile, and 24 chemiluminescence detection reactions with CSPD, ready-to-use. 碩嘉生物常年現貨銷售roche的地高辛DIG標記檢測試劑盒KitII(化學發光),歡迎廣大客戶來電咨詢!


    DIG-High Prime DNA Labeling and Detection Starter Kit II  的詳細介紹

    DIG-High Prime DNA Labeling and Detection Starter Kit II

    roche的地高辛DIG標記檢測試劑盒KitII(化學發光)

    Sample Materials

    ·                                 DNA fragments of at least 100 bp

    ·                                 Linearized plasmid, cosmid or λDNA

    ·                                 Supercoiled DNA

    Sensitivity and specificity: Standard assay uses 1 μg of linearized pBR328. It is usual to expect 0.8 μg labeled DNA after 1 hour of labeling, and 2.3 μg labeled DNA after 20 hours reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 μg of digested placenta DNA.
     

    Principle

    The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3'-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.
     

    Quality

    Function tested in a dot blot.

     

    上海碩嘉生物常年現貨銷售DIG-High Prime DNA Labeling and Detection Starter Kit II,roche的地高辛DIG標記檢測試劑盒KitII(化學發光),2015年全年促銷該產品,歡迎廣大客戶來電咨詢!

    DIG-High Prime DNA Labeling and Detection Starter Kit II

    DIG-High Prime DNA Labeling and Detection Starter Kit II

    DIG-High Prime DNA Labeling and Detection Starter Kit II

     

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